PC-12 nuclear extract lysate (forskolin stimulated) (ab14885)
Overview
-
Product name
PC-12 nuclear extract lysate (forskolin stimulated)
See all PC12 lysates -
General notes
The protein content has been determined by a Bradford-based assay. The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.37% PBS, 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitor -
Concentration information loading...
-
Research areas
-
Background
Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab14885 has not yet been referenced specifically in any publications.