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Recombinant protein of Human PC1/3 protein (NP_000430.3).
Our Abpromise guarantee covers the use of ab117735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 84 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute @ RT and proceed with staining.|
|ICC/IF||Use a concentration of 30 µg/ml.|
ab117735 has not yet been referenced specifically in any publications.