Overview

  • Product nameAnti-PC1/3 antibody
    See all PC1/3 primary antibodies
  • Description
    Mouse monoclonal to PC1/3
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 652-754 of Human PC1/3

Properties

Applications

Our Abpromise guarantee covers the use of ab55543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Detection limit for recombinant GST-tagged PCSK1 is approximately 0.03 ng/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 84 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 30 µg/ml.

Target

  • FunctionInvolved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
  • Involvement in diseaseDefects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
  • Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDP antibody
    • NEC 1 antibody
    • NEC1 antibody
    • NEC1_HUMAN antibody
    • Neuroendocrine convertase 1 antibody
    • PC1 antibody
    • PC3 antibody
    • PCSK1 antibody
    • Prohormone convertase 1 antibody
    • Prohormone convertase 1/3 antibody
    • Prohormone convertase 3 antibody
    • Proprotein convertase 1 antibody
    • SPC3 antibody
    see all

Anti-PC1/3 antibody images

  • PC1/3 antibody (ab55543) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 84 kDa
  • ab55543 at 30 µg/ml staining PC1/3 in HeLa cells by Immunocytochemistry/ Immunofluorescense.
  • Anti-PC1/3 antibody (ab55543) + PC-12 cells

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • Anti-PC1/3 antibody (ab55543) + Raw 264.7 cell lysate

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • Anti-PC1/3 antibody (ab55543) + NIH/3T3 cell lysate

    Predicted band size : 84 kDa
    Observed band size : 84 kDa

References for Anti-PC1/3 antibody (ab55543)

This product has been referenced in:
  • Moffett RC  et al. Incretin Receptor Null Mice Reveal Key Role of GLP-1 but Not GIP in Pancreatic Beta Cell Adaptation to Pregnancy. PLoS One 9:e96863 (2014). IHC-P ; Mouse . Read more (PubMed: 24927416) »
  • O'Brien M  et al. Ghrelin in the human myometrium. Reprod Biol Endocrinol 8:55 (2010). WB ; Human . Read more (PubMed: 20509935) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry and I am sorry to hear you are having difficulty with ab55543. Unfortunately, ab55543 has not been tested in mouse, so we are unsure how or if it will work in this species.  This antibody is only guaranteed to work in hum...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"