Overview

  • Product nameAnti-PC4 antibody
    See all PC4 primary antibodies
  • Description
    Rabbit polyclonal to PC4
  • Specificityab23594 reacts with PC4 (activated RNA polymerase II transcription cofactor 4).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 73 - 127 of Human PC4.

  • Positive control
    • HepG2 cell lysate for WB Human Liver for IHC-p

Properties

Applications

Our Abpromise guarantee covers the use of ab23594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
  • Post-translational
    modifications
    Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activated RNA polymerase II transcriptional coactivator p15 antibody
    • Interferon related developmental regulator 1 antibody
    • MGC102747 antibody
    • p14 antibody
    • P15 antibody
    • PC4 antibody
    • PC4 LSB antibody
    • Positive cofactor 4 antibody
    • RPO2TC1 antibody
    • Sub1 antibody
    • SUB1 homolog antibody
    • TCP4_HUMAN antibody
    see all

Anti-PC4 antibody images

  • Anti-PC4 antibody (ab23594) at 2 µg/ml + HepG2 cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling PC4 with ab23594 at a concentration of 4-8µg/ml. Positive cells marked by arrows are hepatocytes. Magnification 400X.

References for Anti-PC4 antibody (ab23594)

ab23594 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"