Overview

  • Product nameAnti-PC4 antibody
    See all PC4 primary antibodies
  • Description
    Rabbit polyclonal to PC4
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residues 77 and 127 of Human PC4 (NP_006704.2)

  • Positive control
    • Human: bladder carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin melanoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: skin, seratoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may improve staining.
    Note: likely to work with frozen sections.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
    • Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
    • Post-translational
      modifications
      Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activated RNA polymerase II transcriptional coactivator p15 antibody
      • Interferon related developmental regulator 1 antibody
      • MGC102747 antibody
      • p14 antibody
      • P15 antibody
      • PC4 antibody
      • PC4 LSB antibody
      • Positive cofactor 4 antibody
      • RPO2TC1 antibody
      • Sub1 antibody
      • SUB1 homolog antibody
      • TCP4_HUMAN antibody
      see all

    Anti-PC4 antibody images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue using 1/250 ab84459, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue using 1/250 ab84459, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma tissue using 1/250 ab84459, followed by DAB staining.

    References for Anti-PC4 antibody (ab84459)

    ab84459 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84459.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"