Anti-PC4 antibody (ab84459)
Key features and details
- Rabbit polyclonal to PC4
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PC4 antibody
See all PC4 primary antibodies -
Description
Rabbit polyclonal to PC4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to a region between residues 77 and 127 of Human PC4 (NP_006704.2)
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Positive control
- Human: bladder carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin melanoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: skin, seratoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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Note: likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). -
Sequence similarities
Belongs to the transcriptional coactivator PC4 family. -
Post-translational
modificationsActivity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10923 Human
- Entrez Gene: 20024 Mouse
- Entrez Gene: 192269 Rat
- Omim: 600503 Human
- SwissProt: P53999 Human
- SwissProt: P11031 Mouse
- SwissProt: Q63396 Rat
- Unigene: 229641 Human
see all -
Alternative names
- Activated RNA polymerase II transcriptional coactivator p15 antibody
- Interferon related developmental regulator 1 antibody
- MGC102747 antibody
see all
Images
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue using 1/250 ab84459, followed by DAB staining.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue using 1/250 ab84459, followed by DAB staining.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma tissue using 1/250 ab84459, followed by DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab84459 has been referenced in 4 publications.
- Ochiai K et al. Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins. STAR Protoc 2:100633 (2021). PubMed: 34258594
- Liao F et al. PC4 serves as a negative regulator of skin wound healing in mice. Burns Trauma 8:tkaa010 (2020). PubMed: 32373645
- Ochiai K et al. Chromatin Protein PC4 Orchestrates B Cell Differentiation by Collaborating with IKAROS and IRF4. Cell Rep 33:108517 (2020). PubMed: 33357426
- Westphal N et al. Nuclear fragments of the neural cell adhesion molecule NCAM with or without polysialic acid differentially regulate gene expression. Sci Rep 7:13631 (2017). PubMed: 29051583