Recombinant
RabMAb

Anti-PC4 antibody [EPR9281] (ab154852)

Overview

  • Product name
    Anti-PC4 antibody [EPR9281]
    See all PC4 primary antibodies
  • Description
    Rabbit monoclonal [EPR9281] to PC4
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PC4 aa 1-100.
    Database link: P53999

  • Positive control
    • MCF7, T47D, Jurkat and PC3 cell lysates; MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
ICC/IF 1/100 - 1/250.

For unpurified, use 1/100 - 1/250.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
    • Sequence similarities
      Belongs to the transcriptional coactivator PC4 family.
    • Post-translational
      modifications
      Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activated RNA polymerase II transcriptional coactivator p15 antibody
      • Interferon related developmental regulator 1 antibody
      • MGC102747 antibody
      • p14 antibody
      • P15 antibody
      • PC4 antibody
      • PC4 LSB antibody
      • Positive cofactor 4 antibody
      • RPO2TC1 antibody
      • Sub1 antibody
      • SUB1 homolog antibody
      • TCP4_HUMAN antibody
      see all

    Images

    • All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : PC-3 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size : 14 kDa
      Observed band size : 14 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 μg Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : T47D cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 14 kDa
    • Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.

    References

    ab154852 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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