Anti-PC6 antibody - Aminoterminal end (ab39873)

Overview

  • Product name
    Anti-PC6 antibody - Aminoterminal end
    See all PC6 primary antibodies
  • Description
    Rabbit polyclonal to PC6 - Aminoterminal end
  • Specificity
    ab39873 recognizes the Amino end of the mature enzyme subtilisin like serine protease PC6.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the amino end of mature Human PC6.

    (Peptide available as ab41413.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: 1/500 (PMID 19339735).
    WB: 1/1000 - 1/5000. Predicted molecular weight: 101 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Likely to represent a widespread endoprotease activity within the constitutive and regulated secretory pathway. Capable of cleavage at the RX(K/R)R consensus motif.
    • Tissue specificity
      Expressed in T-lymphocytes.
    • Sequence similarities
      Belongs to the peptidase S8 family.
      Contains 1 homo B/P domain.
      Contains 1 PLAC domain.
    • Domain
      The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ11149 antibody
      • FLJ16215 antibody
      • hPC6 antibody
      • PC5 antibody
      • PC6 antibody
      • PC6A antibody
      • Pcsk5 antibody
      • PCSK5_HUMAN antibody
      • Prohormone convertase 5 antibody
      • Proprotein convertase 5 antibody
      • Proprotein convertase 6 antibody
      • Proprotein convertase PC5 antibody
      • Proprotein convertase subtilisin/kexin type 5 antibody
      • Protease PC6 antibody
      • SPC6 antibody
      • Subtilisin/kexin-like protease PC5 antibody
      see all

    References

    This product has been referenced in:
    • Sathyamurthy M  et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . Read more (PubMed: 25219685) »
    • Zhou Z  et al. The proprotein convertase furin is required for trophoblast syncytialization. Cell Death Dis 4:e593 (2013). WB ; Mouse, Human . Read more (PubMed: 23598405) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (pancreatic cancer cells)
    Permeabilization
    Yes - 0.1% Tween20
    Specification
    pancreatic cancer cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1%
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 15 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat ovary primary cultured cell)
    Loading amount
    50 µg
    Specification
    rat ovary primary cultured cell
    Treatment
    LH hormone
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 31 2012

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untes...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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