PC6 antibody - Homp-P domain (ab40133)

Product Name 

PC6 antibody - Homp-P domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PC6 - Homp-P domain

Immunogen 

Synthetic peptide based on the Homp-P Domain of human PC6.

(Peptide available as ab41414.)

Reacts with 
(species key)

Hu

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all PC6 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, 1/5000 for chemiluminescent substrates. Predicted molecular weight: 102 kDa. Ab40133 detects Pre-pro PC6 at 117 kDa (this is higher than the predicted molecular weight due to glycosylation and other post-translational modifications) and the mature PC6 at 65 kDa. Smaller cleavage products are also detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases

Nuclear Signaling >> DNA / RNA >> Translation >> ER Translocation

Relevance 

PC6 (or PC5) belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. PC6 mediates posttranslational endoproteolytic processing for several integrin alpha subunits. It is thought to process prorenin, pro-membrane type 1 matrix metalloproteinase and HIV1 glycoprotein gp160. Two splice variants are reported; the original 913 amino acid sequence (PC5A) and an 1877 amino acid membrane-associated form (PC5B). The longer form is thought to be bound in the Golgi, and the shorter form secreted through dense core secretory granules. PC6 has been shown to cleave the neural adhesion protein L1, Preo-neurotension, VEGF-C, Integrin alpha V, some viral surface proteins, and is thought to process prorenin. Like furin, PC2 and PACE4, PC6 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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