Overview

  • Product nameAnti-PCB antibody
    See all PCB primary antibodies
  • Description
    Goat polyclonal to PCB
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cow, Dog, Human, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-KFKEVKKAYVEANQ

    , corresponding to internal sequence amino acids 886-899 of Human PCB (NP_000911.2; NP_001035806.1; NP_071504.2).

  • Positive control
    • Mouse Liver lysate.
  • General notesAll reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
    • PathwayCarbohydrate biosynthesis; gluconeogenesis.
    • Involvement in diseaseDefects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
    • Sequence similaritiesContains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • mitochondrial antibody
      • OTTHUMP00000235155 antibody
      • OTTHUMP00000235156 antibody
      • PC antibody
      • PCB antibody
      • Pcx antibody
      • PYC_HUMAN antibody
      • Pyruvate carboxylase antibody
      • Pyruvate carboxylase mitochondrial antibody
      • Pyruvic carboxylase antibody
      see all

    Anti-PCB antibody images

    References for Anti-PCB antibody (ab77376)

    This product has been referenced in:
    • Jornayvaz FR  et al. Thyroid hormone receptor-a gene knockout mice are protected from diet-induced hepatic insulin resistance. Endocrinology 153:583-91 (2012). WB ; Mouse . Read more (PubMed: 22147010) »
    • Jornayvaz FR  et al. A high-fat, ketogenic diet causes hepatic insulin resistance in mice, despite increasing energy expenditure and preventing weight gain. Am J Physiol Endocrinol Metab 299:E808-15 (2010). WB ; Mouse . Read more (PubMed: 20807839) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab77376.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"