The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionInvolved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Regulates the dimerization of homeodomain protein HNF-1-alpha and enhances its transcriptional activity.
Sequence similaritiesBelongs to the pterin-4-alpha-carbinolamine dehydratase family.