Anti-PCBP1 antibody (ab109577)
Key features and details
- Goat polyclonal to PCBP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCBP1 antibody
See all PCBP1 primary antibodies -
Description
Goat polyclonal to PCBP1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog -
Immunogen
Synthetic peptide: LDLAKLNQVARQQSH by a Cysteine residue linker, corresponding to internal sequence amino acids 234-247 of Human PCBP1
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Positive control
- HeLa and Human Placenta lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. -
Tissue specificity
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsPhosphorylated; lowers poly(rC)-binding activity. -
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5093 Human
- Entrez Gene: 23983 Mouse
- Entrez Gene: 500242 Rat
- Omim: 601209 Human
- SwissProt: Q5E9A3 Cow
- SwissProt: Q15365 Human
- SwissProt: P60335 Mouse
- SwissProt: O19048 Rabbit
see all -
Alternative names
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
see all
Images
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Anti-PCBP1 antibody (ab109577) at 0.01 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab109577 has been referenced in 1 publication.
- Zheng Y et al. The RNA-binding protein PCBP1 represses lung adenocarcinoma progression by stabilizing DKK1 mRNA and subsequently downregulating β-catenin. J Transl Med 20:343 (2022). PubMed: 35907982