Overview

  • Product nameAnti-PCBP1 antibody
    See all PCBP1 primary antibodies
  • Description
    Goat polyclonal to PCBP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide:

    SIQGQHTISPLD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 223-234 of Human PCBP1(NP_006187.2).

  • Positive control
    • Human Peripheral Blood Nucleocytes lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).

Target

  • FunctionSingle-stranded nucleic acid binding protein that binds preferentially to oligo dC.
  • Tissue specificityAbundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    modifications
    Phosphorylated; lowers poly(rC)-binding activity.
  • Cellular localizationNucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-CP1 antibody
    • Heterogeneous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein X antibody
    • hnRNP E1 antibody
    • hnRNP-E1 antibody
    • hnRNP-X antibody
    • HNRPE1 antibody
    • HNRPX antibody
    • nucleic acid binding protein sub 2.3 antibody
    • Nucleic acid- binding protein SUB2.3 antibody
    • Nucleic acid-binding protein SUB2.3 antibody
    • PCBP1 antibody
    • PCBP1_HUMAN antibody
    • poly(rC) binding protein 1 antibody
    • Poly(rC)-binding protein 1 antibody
    • rCbinding protein 1 antibody
    see all

Anti-PCBP1 antibody images

  • All lanes : Anti-PCBP1 antibody (ab109578) at 0.10000000149 µg/ml

    Lane 1 : Rat spleen lysate
    Lane 2 : Rat thymus lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 37 kDa
  • Anti-PCBP1 antibody (ab109578) at 0.1 µg/ml + Human Peripheral Blood Nucleocytes lysates (in RIPA buffer)) at 35 µg
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-PCBP1 antibody (ab109578)

ab109578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"