The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 mg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Contains 3 KH domains.
Phosphorylated; lowers poly(rC)-binding activity.
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
Western blot - Anti-PCBP1 antibody (ab74793)Image courtesy of an anonymous Abreview.
All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells Lane 2 : Whole cell lysate prepared from F9 cells Lane 3 : Whole cell lysate prepared from GC1 cells Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 30 µg per lane.
Secondary HRP conjugated pig anti-rabbit polyclonal Developed using the ECL technique
Ho JJ et al. Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs. Mol Cell Biol33:2029-46 (2013).
Read more (PubMed: 23478261) »
Sutherland JM et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol227:1188-98 (2012).
Read more (PubMed: 21604262) »