Overview

  • Product name
    Anti-PCBP1 antibody [EPR11049(B)]
    See all PCBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11049(B)] to PCBP1
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PCBP1 (UniProt ID: Q15365).

  • Positive control
    • HeLa, HepG2, K562 and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab168377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
    • Tissue specificity
      Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
    • Sequence similarities
      Contains 3 KH domains.
    • Post-translational
      modifications
      Phosphorylated; lowers poly(rC)-binding activity.
    • Cellular localization
      Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-CP1 antibody
      • Heterogeneous nuclear ribonucleoprotein E1 antibody
      • heterogenous nuclear ribonucleoprotein E1 antibody
      • heterogenous nuclear ribonucleoprotein X antibody
      • hnRNP E1 antibody
      • hnRNP-E1 antibody
      • hnRNP-X antibody
      • HNRPE1 antibody
      • HNRPX antibody
      • nucleic acid binding protein sub 2.3 antibody
      • Nucleic acid- binding protein SUB2.3 antibody
      • Nucleic acid-binding protein SUB2.3 antibody
      • PCBP1 antibody
      • PCBP1_HUMAN antibody
      • poly(rC) binding protein 1 antibody
      • Poly(rC)-binding protein 1 antibody
      • rCbinding protein 1 antibody
      see all

    Images

    • All lanes : Anti-PCBP1 antibody [EPR11049(B)] (ab168377) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
    • Western blot analysis on HeLa cell lysate pellet immunoprecipitated using ab168377 at a 1/10 dilution, and an HRP-conjugated anti-rabbit IgG.

    References

    This product has been referenced in:
    • Horiguchi H  et al. Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms. Haematologica 101:437-47 (2016). Read more (PubMed: 26802051) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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