Recombinant
RabMAb

Anti-PCBP1 antibody [EPR11055] (ab168378)

Overview

  • Product name
    Anti-PCBP1 antibody [EPR11055]
    See all PCBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11055] to PCBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PCBP1 (UniProt: Q15365).

  • Positive control
    • HeLa, 293T, HepG2 and K562 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
    • Tissue specificity
      Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
    • Sequence similarities
      Contains 3 KH domains.
    • Post-translational
      modifications
      Phosphorylated; lowers poly(rC)-binding activity.
    • Cellular localization
      Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-CP1 antibody
      • Heterogeneous nuclear ribonucleoprotein E1 antibody
      • heterogenous nuclear ribonucleoprotein E1 antibody
      • heterogenous nuclear ribonucleoprotein X antibody
      • hnRNP E1 antibody
      • hnRNP-E1 antibody
      • hnRNP-X antibody
      • HNRPE1 antibody
      • HNRPX antibody
      • nucleic acid binding protein sub 2.3 antibody
      • Nucleic acid- binding protein SUB2.3 antibody
      • Nucleic acid-binding protein SUB2.3 antibody
      • PCBP1 antibody
      • PCBP1_HUMAN antibody
      • poly(rC) binding protein 1 antibody
      • Poly(rC)-binding protein 1 antibody
      • rCbinding protein 1 antibody
      see all

    Images

    • All lanes : Anti-PCBP1 antibody [EPR11055] (ab168378) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 37 kDa

    • Detection of PCBP1 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab168378 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab168378 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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