Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] (ab61334)

Overview

  • Product nameAnti-PCBP2/hnRNP E2 antibody [376CT13.1.2]
    See all PCBP2/hnRNP E2 primary antibodies
  • Description
    Mouse monoclonal [376CT13.1.2] to PCBP2/hnRNP E2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human PCBP2 protein.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; HepG2; Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab61334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Abcam reccomends blocking with 3% milk.

Target

  • FunctionSingle-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
  • Tissue specificityDetected in all tissues examined.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    modifications
    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
  • Cellular localizationNucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CP2 antibody
    • Alpha-CP2 antibody
    • alphaCP-2 antibody
    • Cbp antibody
    • CTBP antibody
    • Heterogeneous nuclear ribonucleoprotein E2 antibody
    • Heterogenous nuclear ribonucleoprotein E2 antibody
    • hnRNP E2 antibody
    • hnRNP-E2 antibody
    • HNRNPE2 antibody
    • Hnrnpx antibody
    • HNRPE2 antibody
    • Hnrpx antibody
    • MGC110998 antibody
    • PCBP2 antibody
    • PCBP2_HUMAN antibody
    • poly(rC) binding protein 2 antibody
    • Poly(rC)-binding protein 2 antibody
    • Putative heterogeneous nuclear ribonucleoprotein X antibody
    • rCbinding protein 2 antibody
    see all

Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] images

  • All lanes : Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] (ab61334) at 5 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 110 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] (ab61334)

ab61334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"