Anti-PCBP2/hnRNP E2 antibody (ab154245)
Key features and details
- Rabbit polyclonal to PCBP2/hnRNP E2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PCBP2/hnRNP E2 antibody
See all PCBP2/hnRNP E2 primary antibodies -
Description
Rabbit polyclonal to PCBP2/hnRNP E2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 85-365 of Human PCBP2/hnRNP E2 (Q15366).
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Positive control
- Jurkat, Raji, K562 and NCI-H929 whole cell lysates. HeLa cells. Human muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0 |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. -
Tissue specificity
Detected in all tissues examined. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsPhosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. -
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5094 Human
- Omim: 601210 Human
- SwissProt: Q15366 Human
- Unigene: 546271 Human
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Alternative names
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
see all
Images
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All lanes : Anti-PCBP2/hnRNP E2 antibody (ab154245) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NCI H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling PCBP2/hnRNP E2 with ab154245 at 1/500. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded HeLa xenograft tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
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Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154245 has not yet been referenced specifically in any publications.