Overview

  • Product name
    Anti-PCBP2/hnRNP E2 antibody
    See all PCBP2/hnRNP E2 primary antibodies
  • Description
    Rabbit polyclonal to PCBP2/hnRNP E2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 85-365 of Human PCBP2/hnRNP E2 (Q15366).

  • Positive control
    • Jurkat, Raji, K562 and NCI-H929 whole cell lysates. HeLa cells. Human muscle tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
  • Tissue specificity
    Detected in all tissues examined.
  • Sequence similarities
    Contains 3 KH domains.
  • Post-translational
    modifications
    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
  • Cellular localization
    Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CP2 antibody
    • Alpha-CP2 antibody
    • alphaCP-2 antibody
    • Cbp antibody
    • CTBP antibody
    • Heterogeneous nuclear ribonucleoprotein E2 antibody
    • Heterogenous nuclear ribonucleoprotein E2 antibody
    • hnRNP E2 antibody
    • hnRNP-E2 antibody
    • HNRNPE2 antibody
    • Hnrnpx antibody
    • HNRPE2 antibody
    • Hnrpx antibody
    • MGC110998 antibody
    • PCBP2 antibody
    • PCBP2_HUMAN antibody
    • poly(rC) binding protein 2 antibody
    • Poly(rC)-binding protein 2 antibody
    • Putative heterogeneous nuclear ribonucleoprotein X antibody
    • rCbinding protein 2 antibody
    see all

Images

  • All lanes : Anti-PCBP2/hnRNP E2 antibody (ab154245) at 1/2000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : NCI H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling PCBP2/hnRNP E2 with ab154245 at 1/500. Lower panel shows costaining with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded HeLa xenograft tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
  • Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.

References

ab154245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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