Anti-PCBP2/hnRNP E2 antibody (ab96169)

Overview

  • Product name
    Anti-PCBP2/hnRNP E2 antibody
    See all PCBP2/hnRNP E2 primary antibodies
  • Description
    Rabbit polyclonal to PCBP2/hnRNP E2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within N-terminal amino acids 1-163 of Human PCBP2/hnRNP E2.

  • Positive control
    • 293T, A431, Jurkat or Raji cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

Properties

Applications

Our Abpromise guarantee covers the use of ab96169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
  • Tissue specificity
    Detected in all tissues examined.
  • Sequence similarities
    Contains 3 KH domains.
  • Post-translational
    modifications
    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
  • Cellular localization
    Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CP2 antibody
    • Alpha-CP2 antibody
    • alphaCP-2 antibody
    • Cbp antibody
    • CTBP antibody
    • Heterogeneous nuclear ribonucleoprotein E2 antibody
    • Heterogenous nuclear ribonucleoprotein E2 antibody
    • hnRNP E2 antibody
    • hnRNP-E2 antibody
    • HNRNPE2 antibody
    • Hnrnpx antibody
    • HNRPE2 antibody
    • Hnrpx antibody
    • MGC110998 antibody
    • PCBP2 antibody
    • PCBP2_HUMAN antibody
    • poly(rC) binding protein 2 antibody
    • Poly(rC)-binding protein 2 antibody
    • Putative heterogeneous nuclear ribonucleoprotein X antibody
    • rCbinding protein 2 antibody
    see all

Images

  • IHC image of PCBP2/hnRNP E2 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96169, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PCBP2/hnRNP E2 antibody (ab96169) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 39 kDa
    10% SDS PAGE

References

ab96169 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, 293T)
Loading amount
25 µg
Specification
HeLa, 293T
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Apr 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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