Recombinant Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2
- Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCBP2/hnRNP E2 antibody [EPR14858]
See all PCBP2/hnRNP E2 primary antibodies -
Description
Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Raji and K562 cell lysates; Human clear cell carcinoma of kidney tissue; Human skin tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14858 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/40 - 1/60.
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ICC/IF |
1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/110.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
1/40 - 1/60. |
ICC/IF
1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/110. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. -
Tissue specificity
Detected in all tissues examined. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsPhosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. -
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5094 Human
- Entrez Gene: 18521 Mouse
- Entrez Gene: 363005 Rat
- Omim: 601210 Human
- SwissProt: Q15366 Human
- SwissProt: Q61990 Mouse
- Unigene: 546271 Human
- Unigene: 236513 Mouse
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Alternative names
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
see all
Images
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All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : K562 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Intracellular flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed)labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab184962 has been referenced in 5 publications.
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Bae DH et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. Biochim Biophys Acta Mol Basis Dis N/A:165844 (2020). PubMed: 32480040
- Liu Y et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420
- Malik N et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun 10:2071 (2019). PubMed: 31061501
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708