The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/110. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/40 - 1/60.
1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa).
1/50 - 1/100.
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
Detected in all tissues examined.
Contains 3 KH domains.
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody.