Overview

  • Product name
  • Description
    Rabbit polyclonal to PCCA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCCA aa 336-365 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P05165

  • Positive control
    • A549 cell line lysate; Human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 80 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway
    Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • Involvement in disease
    Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • Sequence similarities
    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Pcca antibody
    • pccA COMPLEMENTATION GROUP antibody
    • PCCA_HUMAN antibody
    • PCCase subunit alpha antibody
    • Propanoyl CoA:carbon dioxide ligase subunit alpha antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit alpha antibody
    • Propionyl CoA carboxylase alpha chain antibody
    • Propionyl CoA carboxylase alpha chain mitochondrial antibody
    • Propionyl CoA carboxylase alpha polypeptide antibody
    • Propionyl coenzyme A carboxylase alpha polypeptide antibody
    • Propionyl-CoA carboxylase alpha chain antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling PCCA with ab170685 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining..

  • Anti-PCCA antibody (ab170685) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size: 80 kDa

References

ab170685 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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