1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
Involvement in disease
Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling PCCA with ab170685 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining..
Western blot - Anti-PCCA antibody (ab170685)
Anti-PCCA antibody (ab170685) at 1/100 dilution + A549 cell line lysate at 35 µg