Overview

  • Product nameAnti-PCCA antibody
    See all PCCA primary antibodies
  • Description
    Rabbit polyclonal to PCCA
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: HVLYYSRQCL MVSRNLGSVG YDPNEKTFDK ILVANRGEIA CRVIRTCKKM, corresponding to amino acids 36-85 of Human PCCA (NP_000273).

  • Positive control
    • PCCA transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • PathwayMetabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • Involvement in diseaseDefects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • Sequence similaritiesContains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Pcca antibody
    • pccA COMPLEMENTATION GROUP antibody
    • PCCA_HUMAN antibody
    • PCCase subunit alpha antibody
    • Propanoyl CoA:carbon dioxide ligase subunit alpha antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit alpha antibody
    • Propionyl CoA carboxylase alpha chain antibody
    • Propionyl CoA carboxylase alpha chain mitochondrial antibody
    • Propionyl CoA carboxylase alpha polypeptide antibody
    • Propionyl coenzyme A carboxylase alpha polypeptide antibody
    • Propionyl-CoA carboxylase alpha chain antibody
    see all

Anti-PCCA antibody images

  • Anti-PCCA antibody (ab80346) at 1 µg/ml (in 5% skim milk / PBS buffer) + PCCA transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PCCA antibody (ab80346)

ab80346 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"