Overview

  • Product nameAnti-PCCB antibody
    See all PCCB primary antibodies
  • Description
    Mouse polyclonal to PCCB
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human PCCB protein (NP_000523.2).

  • Positive control
    • PCCB transfected 293T lysate and human liver tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • PathwayMetabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • Involvement in diseaseDefects in PCCB are the cause of propionic acidemia type II (PA-2) [MIM:606054]. PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • Sequence similaritiesBelongs to the AccD/PCCB family.
    Contains 1 carboxyltransferase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp451E113 antibody
    • PCCase subunit beta antibody
    • pccB antibody
    • PCCB_HUMAN antibody
    • pccBC Complementation group antibody
    • Propanoyl CoA:carbon dioxide ligase subunit beta antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit beta antibody
    • Propionyl CoA carboxylase beta chain, mitochondrial antibody
    • propionyl Coenzyme A carboxylase, beta polypeptide antibody
    • Propionyl-CoA carboxylase , beta subunit antibody
    • Propionyl-CoA carboxylase beta chain, mitochondrial antibody
    • R74805 antibody
    see all

Anti-PCCB antibody images

  • Anti-PCCB antibody (ab70416) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PCCB antibody (ab70416) at 1/500 dilution

    Lane 1 : PCCB transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab70416 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PCCB antibody (ab70416)

ab70416 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call today and for letting us know about the trouble with this antibody.
As we discussed, I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow. Please keep me updated about the results ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"