Anti-PCDH1 antibody - N-terminal (ab170812)

Overview

  • Product name
    Anti-PCDH1 antibody - N-terminal
    See all PCDH1 primary antibodies
  • Description
    Rabbit polyclonal to PCDH1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCDH1 aa 186-214 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q08174-2

  • Positive control
    • MDA-MB453, ZR-75-1 lysate, Human brain tissue, U-251MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 133 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Function
    May be involved in cell-cell interaction processes and in cell adhesion.
  • Tissue specificity
    Highly expressed in the brain and neuro-glial cells.
  • Sequence similarities
    Contains 7 cadherin domains.
  • Developmental stage
    Highest expression in adults.
  • Cellular localization
    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin like 1 antibody
    • Cadherin like protein 1 antibody
    • Cadherin-like protein 1 antibody
    • FLJ53887 antibody
    • MGC45991 antibody
    • OTTHUMP00000160067 antibody
    • PC 42 antibody
    • PC42 antibody
    • PCDH 1 antibody
    • PCDH 42 antibody
    • PCDH1 antibody
    • PCDH1_HUMAN antibody
    • PCDH42 antibody
    • Protocadherin 1 antibody
    • Protocadherin 42 antibody
    • Protocadherin-1 antibody
    • Protocadherin-42 antibody
    • Protocadherin1 antibody
    • Protocadherin42 antibody
    see all

Images

  • Confocal immunofluorescent analysis of U-251MG cells using ab170812 at a 1/10 dilution, followed by a goat anti-rabbit lgG conjugated to dye (green). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-PCDH1 antibody - N-terminal (ab170812) at 1/100 dilution

    Lane 1 : MDA-MB453 cell line lysates
    Lane 2 : ZR-75-1 cell line lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 133 kDa

  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PCDH1 with ab170812 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170812 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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