Recombinant Anti-PCDH11X antibody [EPR16916] (ab203520)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16916] to PCDH11X
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PCDH11X antibody [EPR16916]
See all PCDH11X primary antibodies -
Description
Rabbit monoclonal [EPR16916] to PCDH11X -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: human hippocampus, heart lysates, mouse hippocampus, cerebellum, thalamus lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16916 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa). |
Target
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Function
Potential calcium-dependent cell-adhesion protein. -
Tissue specificity
Expressed strongly in fetal brain and brain (cortex, amygdala, thalamus, substantia nigra, hippocampus, caudate nucleus and corpus callosum). Expressed at low level in testis. -
Sequence similarities
Contains 7 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27328 Human
- Entrez Gene: 245578 Mouse
- Omim: 300246 Human
- SwissProt: Q9BZA7 Human
- Unigene: 655673 Human
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Alternative names
- PC11X_HUMAN antibody
- PCDH-X antibody
- PCDH11 antibody
see all
Images
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All lanes : Anti-PCDH11X antibody [EPR16916] (ab203520) at 1/1000 dilution
Lane 1 : Human Hippocampus lysate
Lane 2 : Human heart lysate
Lane 3 : Human kidney lysate
Lane 4 : Human spleen lysate
Lane 5 : Mouse hippocampus lysate
Lane 6 : Mouse cerebellum lysate
Lane 7 : Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 148 kDa
Exposure time: 180 secondsBlocking and diluting buffer:
5% NFDM/TBST
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All lanes : Anti-PCDH11X antibody [EPR16916] (ab203520) at 1/1000 dilution
Lane 1 : Mouse thalamus lysate
Lane 2 : Mouse hippocampus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 148 kDa
Observed band size: 148 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab203520 has not yet been referenced specifically in any publications.