Overview

  • Product name
    Anti-PCDH12 antibody
    See all PCDH12 primary antibodies
  • Description
    Rabbit polyclonal to PCDH12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 612-661 (SSRPFLLTTI VARDADSGAN GEPLYSIRSG NEAHLFILNP HTGQLFVNVT) of human PCDH12 (NP_057664).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton.
  • Tissue specificity
    Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression. Prominent expression in the spleen.
  • Sequence similarities
    Contains 6 cadherin domains.
  • Cellular localization
    Cell membrane. Cell junction.
  • Information by UniProt
  • Database links
    • Alternative names
      • PCD12_HUMAN antibody
      • Pcdh12 antibody
      • Protocadherin 12 antibody
      • Protocadherin-12 antibody
      • Vascular cadherin 2 antibody
      • Vascular cadherin-2 antibody
      • Vascular endothelial cadherin 2 antibody
      • Vascular endothelial cadherin-2 antibody
      • VE cadherin 2 antibody
      • VE-cad-2 antibody
      • VE-cadherin-2 antibody
      • VECAD2 antibody
      see all

    Images

    • Western blot - PCDH12 antibody (ab82885)
      Western blot - PCDH12 antibody (ab82885)
      Anti-PCDH12 antibody (ab82885) at 1 µg/ml + MCF7 cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 129 kDa
      Observed band size: 143 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 45 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

    References

    ab82885 has not yet been referenced specifically in any publications.

    Publishing research using ab82885? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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