Overview

  • Product name
  • Description
    Rabbit polyclonal to PCDH12
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 612-661 (SSRPFLLTTI VARDADSGAN GEPLYSIRSG NEAHLFILNP HTGQLFVNVT) of human PCDH12 (NP_057664).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton.
  • Tissue specificity
    Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression. Prominent expression in the spleen.
  • Sequence similarities
    Contains 6 cadherin domains.
  • Cellular localization
    Cell membrane. Cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • PCD12_HUMAN antibody
    • Pcdh12 antibody
    • Protocadherin 12 antibody
    • Protocadherin-12 antibody
    • Vascular cadherin 2 antibody
    • Vascular cadherin-2 antibody
    • Vascular endothelial cadherin 2 antibody
    • Vascular endothelial cadherin-2 antibody
    • VE cadherin 2 antibody
    • VE-cad-2 antibody
    • VE-cadherin-2 antibody
    • VECAD2 antibody
    see all

Images

  • Anti-PCDH12 antibody (ab82885) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 129 kDa
    Observed band size : 143 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References

ab82885 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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