Tissue specificityModerately expressed in all regions of the brain examined, with lowest levels found in the cerebellum. Moderate expression is also found in ovary, and low expression in all other tissues tested. Also detected in primary skin fibroblast.
Involvement in diseaseEpileptic encephalopathy, early infantile, 9
Sequence similaritiesContains 6 cadherin domains.
Developmental stageExpressed in developing cortical plate, amygdala and subcortical regions and in the ganglionic eminence.
Western blot against tagged recombinant protein immunogen using ab57510 PCDH19 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Flow Cytometry-Anti-PCDH19 antibody(ab57510)
Overlay histogram showing SH-SY5Y cells stained with ab57510 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57510, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-PCDH19 antibody (ab57510)
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