Anti-PCDH7 antibody (ab55506)

Overview

  • Product nameAnti-PCDH7 antibody
    See all PCDH7 primary antibodies
  • Description
    Mouse monoclonal to PCDH7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Xenopus laevis, Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGEVTFSLES GSEYLKIDNL TGELSTSERR IDREKLPQCQ MIFDENECFL DFEVSVIGPS QSWV, corresponding to amino acids 31-125 of Human PCDH7

Properties

Applications

Our Abpromise guarantee covers the use of ab55506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityExpressed predominantly in brain and heart and at lower levels in various other tissues.
    • Sequence similaritiesContains 7 cadherin domains.
    • Post-translational
      modifications
      Isoform C is phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BH Pcdh antibody
      • BH protocadherin antibody
      • BH-Pcdh antibody
      • BHPCDH antibody
      • Brain heart protocadherin antibody
      • Brain-heart protocadherin antibody
      • PCDH 7 antibody
      • PCDH7 antibody
      • PCDH7_HUMAN antibody
      • Protocadherin 7 antibody
      • Protocadherin-7 antibody
      see all

    Anti-PCDH7 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55506 PCDH7 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    References for Anti-PCDH7 antibody (ab55506)

    ab55506 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you for your reply and the additional information.
    I have one last question:
    Since a truncated PCDH7 construct (+ GFP) was used, I assume the construct contained the antibody binding site (immunogen: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGE...

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    Thank you for sending the protocol details. This very helpful.
    I have a few additional questions:
    1) At what MW was the band for GFP-PCDH7 seen?
    2) Does the secondary antibody work well with other primaries?
    3) What lysis buffer was...

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    Thank you for contacting us.
    I am sorry to hear the antibody is not working as expected.
    As we discussed over the phone, can you please send me the WB image as well as protocol information you have from your colleagues, so that we can evaluat...

    Read More

    Thank you for your call today.

    As we discussed, please let me know which of these options you would prefer, and I'll be happy to help you further.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Xenopus laevis Tissue lysate - whole (Eye)
    Loading amount 15 µg
    Specification Eye
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    Verified customer

    Submitted Nov 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"