The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/62500.
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevancePCDHA3 contains six cadherin domains. It is thought to be a calcium dependent cell adhesion protein and may be involved in the establishment and maintenance of specific neuronal connections in the brain. There are two named isoforms.
Cellular localizationCell membrane; Single pass type I membrane protein.