The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevancePCDHA5 (Protocadherin alpha 5) is a member of the protocadherin family, a subgroup of the cadherin superfamily. Protocadherins are neural cadherin like cell surface proteins which are present at synaptic junctions. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific neuronal connections in the brain. PCDHA5 is one of fifteen tandemly arranged genes within the alpha cluster of protocadherin genes on 5q31.
Cellular localizationCell membrane; Single pass type I membrane protein.