Anti-PCDHA8 antibody - C-terminal (ab170788)

Overview

  • Product name
    Anti-PCDHA8 antibody - C-terminal
  • Description
    Rabbit polyclonal to PCDHA8 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCDHA8 aa 780-808 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y5H6

  • Positive control
    • ZR-75-1 cells; ZR-75-1 cell lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 103 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

Target

  • Relevance
    PCDHA8 is most likely to play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. Two different isoforms are known.
  • Cellular localization
    Cell membrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • KIAA0345 like 6 antibody
    • PCDH ALPHA8 antibody
    • Protocadherin alpha 8 antibody

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling PCDHA8 with ab170788 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal immunofluorescent analysis of ZR-75-1 cells labeling PCDHA8 with ab170788 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-PCDHA8 antibody - C-terminal (ab170788) at 1/100 dilution + ZR-75-1 cell lysate at 35 µg

    Predicted band size : 103 kDa

References

ab170788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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