Overview

  • Product name
  • Description
    Rabbit polyclonal to PCDHAC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCDHAC2 aa 623-652 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y5I4

  • Positive control
    • NCI-H460 cell line lysates. Human brain tissue. NCI-H460 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170796 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 109 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    PCDHAC2 (Protocadherin alpha C2) is a member of the protocadherin family, a subgroup of the cadherin superfamily. Protocadherins are neural cadherin-like cell adhesion proteins that are integral membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific neuronal connections in the brain. PCDHAC2 is one of fifteen tandemly arranged genes within the alpha cluster of protocadherin genes on 5q31.
  • Cellular localization
    Cell membrane. Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • MGC71598 antibody
    • PCDH alpha C2 antibody
    • PCDH-ALPHA-C2 antibody
    • PCDHAC 2 antibody
    • Protocadherin alpha C2 antibody
    • Protocadherin alpha subfamily C 2 antibody
    • Protocadherin-alpha, subfamily C, member 2 antibody
    see all

Images

  • Flow cytometric analysis of NCI-H460 cells labeling PCDHAC2 using ab170796 at a 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used.

  • Immunocytochemistry/ Immunofluorescence analysis of NCI-H460 cells labeling PCDHAC2 with ab170796 at a 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human brain tissue labeling PCDHAC2 with ab170796 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-PCDHAC2 antibody (ab170796) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size: 109 kDa

References

ab170796 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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