The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceProtocadherin beta 15 (PCDHB15) is a member of the protocadherin family. Protocadherins constitute the largest subgroup within the cadherin family of calcium-dependent cell-cell adhesion molecules. They are neural cell surface proteins which are present at synaptic junctions. Their specific functions are unknown but they may be involved in the establishment and function of specific neuronal connections in the brain. PCDHB15 is one of sixteen tandemly arranged genes in the PCDHB gene cluster on chromosome 5q31.
Cellular localizationCell membrane; Single-pass type I membrane protein.