Overview

  • Product name
  • Description
    Goat polyclonal to PCID1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    NAWKQNLNKVKN

    by a Cysteine residue linker, corresponding to C terminal amino acids 355-366 of Human PCID1 (NP_006351.2).

  • Positive control
    • A549 lysate; Human Testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 43 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
  • Tissue specificity
    Broadly expressed.
  • Sequence similarities
    Belongs to the eIF-3 subunit M family.
    Contains 1 PCI domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B5 antibody
    • B5 receptor antibody
    • Dendritic cell protein antibody
    • eIF3m antibody
    • EIF3M_HUMAN antibody
    • Eukaryotic translation initiation factor 3 subunit M antibody
    • Fetal lung protein B5 antibody
    • FLJ29030 antibody
    • GA17 antibody
    • hfl B5 antibody
    • hFL-B5 antibody
    • PCI domain containing 1 (herpesvirus entry mediator) antibody
    • PCI domain-containing protein 1 antibody
    • PCID1 antibody
    • TANGO7 antibody
    see all

Images

  • Anti-PCID1 antibody (ab115585) at 0.1 µg/ml + A549 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa
  • ab115585, at 5 µg/ml, staining PCID1 in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115585 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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