Overview

  • Product nameAnti-PCK1 antibody
    See all PCK1 primary antibodies
  • Description
    Goat polyclonal to PCK1
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    HVNWFRKDKEGK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 513-524 of Human PCK1 (NP_002582.3).

  • Positive control
    • Human kidney and liver tissues.

Applications

Our Abpromise guarantee covers the use of ab115693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificityMajor sites of expression are liver, kidney and adipocytes.
  • PathwayCarbohydrate biosynthesis; gluconeogenesis.
  • Involvement in diseaseDefects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similaritiesBelongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK1 antibody images

  • ab115693, at 2µg/ml, staining PCK1 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115693, at 2µg/ml, staining PCK1 in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-PCK1 antibody (ab115693)

ab115693 has not yet been referenced specifically in any publications.

Product Wall

Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"