Overview

  • Product nameAnti-PCK1 antibody
    See all PCK1 primary antibodies
  • Description
    Rabbit polyclonal to PCK1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Monkey
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans
  • Immunogen

    Synthetic peptide derived from within residues 330 - 379 of human PEP Carboxylase.

  • Positive control
    • Jurkat cell lysate, Human kidney

Applications

Our Abpromise guarantee covers the use of ab28455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificityMajor sites of expression are liver, kidney and adipocytes.
  • PathwayCarbohydrate biosynthesis; gluconeogenesis.
  • Involvement in diseaseDefects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similaritiesBelongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK1 antibody images



  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate


  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Human MCF7 cell Lysate


  • Predicted band size : 69 kDa
    Western Blot using ab28455 at 1.0ug/ml. Sample type: Human Jurkat cell Lysate
  • Anti-PCK1 antibody (ab28455) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1:50,000-100,000

    Predicted band size : 69 kDa
  • Antibody concentration: 4 - 8 ug/ml Paraffin-embeded Tissue: Human Kidney Cells with positive label: Epithelial cells of renal tubule (indicated with arrows) Magnification: x400
  • ICC/IF image of ab28455 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28455, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PCK1 antibody (ab28455)

This product has been referenced in:
  • Li B  et al. Fructose-1,6-bisphosphatase opposes renal carcinoma progression. Nature 513:251-5 (2014). Read more (PubMed: 25043030) »
  • Jakobs HH  et al. The N-Reductive System Composed of Mitochondrial Amidoxime Reducing Component (mARC), Cytochrome b5 (CYB5B) and Cytochrome b5 Reductase (CYB5R) Is Regulated by Fasting and High Fat Diet in Mice. PLoS One 9:e105371 (2014). WB ; Mouse . Read more (PubMed: 25144769) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Sheep Tissue lysate - whole (Fetal and Maternal Liver)
Loading amount 100 µg
Specification Fetal and Maternal Liver
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% Tris/Glycine-SDS)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rob Cordery-Cotter

Verified customer

Submitted Nov 21 2012

Thank you for your phone call.

DISCOUNT CODE: xxx for ab28455, Value: xxx
DISCOUNT CODE: xxx for ab83690, Value: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab28455 and ...

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I am very pleased to hear you would like to test ab28455, ab83690, and ab138003 in sheep. Each code will give you for value listed above off a future order. To redeem this offer, please submit an Abreview for sheep for each antibody and include the cod...

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Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps test...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Monkey Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Hepatoma cell line (H4IIE))
Loading amount 50 µg
Specification Hepatoma cell line (H4IIE)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Prof. Jianping Ye

Verified customer

Submitted Mar 29 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hepatocytes (HepG2))
Loading amount 50 µg
Specification hepatocytes (HepG2)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Prof. Jianping Ye

Verified customer

Submitted Mar 29 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"