Overview

  • Product nameAnti-PCK1 antibody
    See all PCK1 primary antibodies
  • Description
    Goat polyclonal to PCK1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide:

    C-EKEVEDIEKYLEDQ

    , corresponding to internal sequence (near C terminus) amino acids 586-599 of Human PCK1

  • Positive control
    • Human Kidney lysate.

Applications

Our Abpromise guarantee covers the use of ab61701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesPeptide ELISA: antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • Tissue specificityMajor sites of expression are liver, kidney and adipocytes.
    • PathwayCarbohydrate biosynthesis; gluconeogenesis.
    • Involvement in diseaseDefects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • Sequence similaritiesBelongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • Post-translational
      modifications
      Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    Anti-PCK1 antibody images

    • Anti-PCK1 antibody (ab61701) at 0.5 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer)

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References for Anti-PCK1 antibody (ab61701)

    This product has been referenced in:
    • Jornayvaz FR  et al. Thyroid hormone receptor-a gene knockout mice are protected from diet-induced hepatic insulin resistance. Endocrinology 153:583-91 (2012). WB ; Mouse . Read more (PubMed: 22147010) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"