Anti-PCK1 antibody (ab87340)

Overview

• Product name
  Anti-PCK1 antibody
  See all PCK1 primary antibodies
• Description
  Rabbit polyclonal to PCK1
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PCK1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab87372.)

• Positive control
  • This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Immunizing Peptide (Blocking)
  • PCK1 peptide (ab217264)
• Positive Controls
  • Recombinant Human PCK1 protein (ab114362)
  • Recombinant Human PCK1 protein (ab119469)

Applications

Target

• Function
  Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
• Tissue specificity
  Major sites of expression are liver, kidney and adipocytes.
• Pathway
  Carbohydrate biosynthesis; gluconeogenesis.
• Involvement in disease
  Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
• Sequence similarities
  Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
• Post-translational
  modifications
  Acetylation is increased on addition of glucose and appears to regulate the protein stability.
• Cellular localization
  Cytoplasm.

• Target information above from: UniProt accession P35558 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 396458 Chicken
  • Entrez Gene: 282855 Cow
  • Entrez Gene: 5105 Human
  • Entrez Gene: 18534 Mouse
  • Entrez Gene: 100144531 Pig
  • Entrez Gene: 362282 Rat
  • Entrez Gene: 378727 Zebrafish
  • Omim: 614168 Human

  • SwissProt: P05153 Chicken
  • SwissProt: P35558 Human
  • SwissProt: Q9Z2V4 Mouse
  • SwissProt: P07379 Rat
  • Unigene: 1872 Human
  • Unigene: 104376 Rat

  see all
• Alternative names
  • cytosolic [GTP] antibody
  • GTP antibody
  • PCK1 antibody

  • PCKGC_HUMAN antibody
  • PEP carboxykinase antibody
  • PEPCK-C antibody
  • PEPCK1 antibody
  • PEPCKC antibody
  • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
  • Phosphoenolpyruvate carboxykinase 1 antibody
  • Phosphoenolpyruvate carboxykinase antibody
  • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
  • Phosphoenolpyruvate carboxykinase, cytosolic antibody
  • Phosphoenolpyruvate carboxylase antibody
  • Phosphopyruvate carboxylase antibody

  see all

Protocols

• Western blot protocols