Overview

  • Product name
    Anti-PCK1 antibody
    See all PCK1 primary antibodies
  • Description
    Rabbit polyclonal to PCK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PCK1.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab87372.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver

Applications

Our Abpromise guarantee covers the use of ab87340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with PCK1 peptide (ab217264).

Target

  • Function
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificity
    Major sites of expression are liver, kidney and adipocytes.
  • Pathway
    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease
    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similarities
    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Images

  • Western blot - Anti-PCK1 antibody (ab87340)
    Western blot - Anti-PCK1 antibody (ab87340)
    All lanes : Anti-PCK1 antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 15 minutes

References

This product has been referenced in:
  • Wang HY  et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J 449:479-89 (2013). Read more (PubMed: 23078342) »

See 1 Publication for this product

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