Recombinant Anti-PCK1/PEPC antibody [EPR6939] (ab125003)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6939] to PCK1/PEPC
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCK1/PEPC antibody [EPR6939]
See all PCK1/PEPC primary antibodies -
Description
Rabbit monoclonal [EPR6939] to PCK1/PEPC -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PCK1/PEPC aa 600-700 (C terminal). The exact sequence is proprietary.
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Positive control
- Human fetal kidney and Human adipose tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6939 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
Tissue specificity
Major sites of expression are liver, kidney and adipocytes. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
Post-translational
modificationsAcetylation is increased on addition of glucose and appears to regulate the protein stability. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5105 Human
- Omim: 614168 Human
- SwissProt: P35558 Human
- Unigene: 1872 Human
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Alternative names
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
Images
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All lanes : Anti-PCK1/PEPC antibody [EPR6939] (ab125003) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125003 has not yet been referenced specifically in any publications.