Recombinant
RabMAb

Anti-PCK1 antibody [EPR6939] (ab125003)

Overview

  • Product name
    Anti-PCK1 antibody [EPR6939]
    See all PCK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6939] to PCK1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids of Human PCK1.

  • Positive control
    • Human fetal kidney and Human adipose tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab125003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • Tissue specificity
      Major sites of expression are liver, kidney and adipocytes.
    • Pathway
      Carbohydrate biosynthesis; gluconeogenesis.
    • Involvement in disease
      Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • Sequence similarities
      Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • Post-translational
      modifications
      Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    Anti-PCK1 antibody [EPR6939] images

    • All lanes : Anti-PCK1 antibody [EPR6939] (ab125003) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human adipose tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-PCK1 antibody [EPR6939] (ab125003)

    ab125003 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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