Overview

  • Product nameAnti-PCK2 antibody
    See all PCK2 primary antibodies
  • Description
    Mouse monoclonal to PCK2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1 - 255 of human PCK2 (AAH01454) with a 26 kDa tag

  • Positive control
    • A-431 cell lysate; immunogen

Applications

Our Abpromise guarantee covers the use of ab77047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • PathwayCarbohydrate biosynthesis; gluconeogenesis.
  • Involvement in diseaseDefects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similaritiesBelongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 4.1.1.32 antibody
    • GTP mitochondrial precursor antibody
    • HGNC:8725 antibody
    • mitochondrial antibody
    • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
    • OTTHUMP00000164700 antibody
    • PCK2 antibody
    • PCKGM_HUMAN antibody
    • PE antibody
    • PEP carboxykinase antibody
    • PEPCK antibody
    • PEPCK deficiency mitochondrial antibody
    • PEPCK M antibody
    • PEPCK-M antibody
    • PEPCK2 antibody
    • Phosphoenolpyruvate carboxykinase [GTP] antibody
    • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
    • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK2 antibody images

  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + recombinant tagged PCK2 protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG(H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 71 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + A-431 cell lysate at 25 µg

    Secondary
    goat anti-kouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa

References for Anti-PCK2 antibody (ab77047)

This product has been referenced in:
  • Leithner K  et al. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24632615) »
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). WB ; Human . Read more (PubMed: 21242281) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"