Overview

  • Product name
  • Description
    Rabbit polyclonal to PCM1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residues 1925 and 1975 of human PCM1.

  • Positive control
    • HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab72443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 270 kDa.
IP Use a concentration of 2 - 5 µg/ml.

Target

  • Function
    Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
  • Tissue specificity
    Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
  • Involvement in disease
    Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
    Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
  • Sequence similarities
    Belongs to the PCM1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCM-1 antibody
    • PCM-1 antibody
    • pcm1 antibody
    • PCM1_HUMAN antibody
    • Pericentriolar material 1 antibody
    • Pericentriolar material 1 protein antibody
    • PTC4 antibody
    see all

Images

  • All lanes : Anti-PCM1 antibody (ab72443) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg


    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
    Lane 1: PCM1 precipitate

    Lane 2: Control IgG

    For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:
  • Schou KB  et al. KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling. Nat Commun 8:14177 (2017). Read more (PubMed: 28134340) »
  • Stephen LA  et al. TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). Elife 4:N/A (2015). ICC . Read more (PubMed: 26386247) »

See all 4 Publications for this product

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