Overview

  • Product name
    Anti-PCM1 antibody [CL0206]
    See all PCM1 primary antibodies
  • Description
    Mouse monoclonal [CL0206] to PCM1
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1384-1478 of Human PCM1.

  • Positive control
    • Human testis, cerebral cortex, fallopian tube and striated muscle tissues; RT4 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab154142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 229 kDa.

Target

  • Function
    Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
  • Tissue specificity
    Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
  • Involvement in disease
    Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
    Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
  • Sequence similarities
    Belongs to the PCM1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPCM-1 antibody
    • PCM-1 antibody
    • pcm1 antibody
    • PCM1_HUMAN antibody
    • Pericentriolar material 1 antibody
    • Pericentriolar material 1 protein antibody
    • PTC4 antibody
    see all

Images

  • Immunohistochemical analysis of Human testis tissue labeling PCM1 with ab154142 at 1/200 dilution.
  • Immunohistochemical analysis of Human cerebral cortex tissue labeling PCM1 with ab154142 at 1/200 dilution.
  • Immunohistochemical analysis of Human fallopian tube tissue labeling PCM1 with ab154142 at 1/200 dilution.
  • Immunohistochemical analysis of Human dtriated muscle tissue labeling PCM1 with ab154142 at 1/200 dilution.
  • Anti-PCM1 antibody [CL0206] (ab154142) at 1/1000 dilution + Human cell line U-251

    Predicted band size: 229 kDa

  • Anti-PCM1 antibody [CL0206] (ab154142) at 1/1000 dilution + RT4 cell lysate

    Predicted band size: 229 kDa

References

This product has been referenced in:
  • Chavali PL  et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun 7:11005 (2016). Read more (PubMed: 26987684) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cell (heart)
Specification
heart
Permeabilization
No
Fixative
acetone and methanol mix
Username

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Verified customer

Submitted Dec 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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