Overview

  • Product nameAnti-PCMF antibody
    See all PCMF primary antibodies
  • Description
    Rabbit polyclonal to PCMF
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePCMF has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.
  • Database links
  • Alternative names
    • DEBT 91 antibody
    • DEBT91 antibody
    • Differentially expressed in branching tubulogenesis 91 antibody
    • DKFZP434L1021 antibody
    • E3 ubiquitin protein ligase KCMF1 antibody
    • FGF induced in gastric cancer antibody
    • FGF induced ubiquitin protein ligase in gastric cancers antibody
    • FIGC antibody
    • KCMF 1 antibody
    • KCMF1 antibody
    • PCMF antibody
    • Potassium channel modulatory factor antibody
    • Potassium channel modulatory factor 1 antibody
    • Zinc finger ZZ domain containing 1 antibody
    • ZZ type zinc finger containing protein 1 antibody
    • ZZZ 1 antibody
    • ZZZ1 antibody
    see all

Anti-PCMF antibody images

  • Anti-PCMF antibody (ab80287) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab80287 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PCMF antibody (ab80287)

ab80287 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"