Overview

  • Product nameAnti-PCMT1 antibody
    See all PCMT1 primary antibodies
  • Description
    Rabbit polyclonal to PCMT1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human PCMT1 (NP_005380)

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: U87 xenograft

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/500.

Target

  • RelevancePCMT1 (Protein-L-isoaspartate (D-aspartate) O-methyltransferase) plays a role in the repair and/or degradation of damaged proteins by initiating the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. It catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • L isoaspartyl protein carboxyl methyltransferase antibody
    • L isoaspartyl/D aspartyl methyltransferase antibody
    • PCMT 1 antibody
    • PIMT antibody
    • Protein beta aspartate methyltransferase antibody
    • Protein L isoaspartate (D aspartate) O methyltransferase antibody
    • Protein L isoaspartyl/D aspartyl methyltransferase antibody
    see all

Anti-PCMT1 antibody images

  • Anti-PCMT1 antibody (ab97446) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 25 kDa
  • Immunohistochemical analysis of PCMT1 in paraffin embedded U87 xenograft, using ab97446 at a 1/500 dilution.

References for Anti-PCMT1 antibody (ab97446)

This product has been referenced in:
  • Yan G  et al. Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1. Int J Cardiol 168:3291-9 (2013). Human . Read more (PubMed: 23647599) »

See 1 Publication for this product

Product Wall

Thank you for your email. I am very sorry it has taken some time to reply, my Spanish colleague Ariana is currently out of the office. The additional blocking step with 0.3% solution of H2O2 in methanol is suitable for HRP detection methods. I would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"