Anti-PCNA antibody [339] (ab12449)

Overview

  • Product nameAnti-PCNA antibody [339]
    See all PCNA primary antibodies
  • Description
    Mouse monoclonal [339] to PCNA
  • SpecificityThis antibody reacts with the interdomain connector loop of human PCNA, other not determined.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant PCNA interdomain connector loop protein (human).

  • Positive control
    • HeLa,293T,PC-12,NIH-3T3,Jurkat,Raji,A431,H1299,MCF-7,NT2D1 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab12449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 31.32 kDa).
ICC/IF 1/500.

Target

  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    modifications
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody [339] images

  • All lanes : Anti-PCNA antibody [339] (ab12449) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : NIH-3T3 whole cell lysate
    Lane 3 : PC-12 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 31.32 kDa

    12% SDS PAGE



  • Predicted band size : 31.32 kDa
    ab12449 staining human PCNA in HeLa whole cell lysate by Western blot (ECL). ab12449 staining human PCNA in HeLa whole cell lysate by Western blot (ECL).
  • All lanes : Anti-PCNA antibody [339] (ab12449) at 1/1000 dilution

    Lane 1 : 293T at 20 µg
    Lane 2 : 293T at 10 µg
    Lane 3 : 293T at 5 µg


    Predicted band size : 31.32 kDa

    12% SDS PAGE

  • All lanes : Anti-PCNA antibody [339] (ab12449) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate at 30 µg
    Lane 2 : Raji whole cell lysate at 30 µg
    Lane 3 : 293T whole cell lysate at 30 µg
    Lane 4 : A431 whole cell lysate at 30 µg
    Lane 5 : HeLa whole cell lysate at 30 µg
    Lane 6 : HepG2 whole cell lysate at 30 µg
    Lane 7 : H1299 whole cell lysate at 30 µg
    Lane 8 : HCT116 whole cell lysate
    Lane 9 : MCF-7 whole cell lysate
    Lane 10 : NT2D1 whole cell lysate
    Lane 11 : PC-3 whole cell lysate
    Lane 12 : U87-MG whole cell lysate at 30 µg


    Predicted band size : 31.32 kDa

    12% SDS PAGE

  • Immunofluorescence aqnalysis of paraformaldehyde-fixed A431,using ab12449 at 1:500 dilution.

References for Anti-PCNA antibody [339] (ab12449)

ab12449 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. From what I have read, a lot of people seem to use both PCNA and BrdU antibodies for comparison when examining cell proliferation. The difference between the two is summed up best in the following publication, the abstract o...

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