Overview

  • Product nameAnti-PCNA antibody [5E6/2]
    See all PCNA primary antibodies
  • Description
    Mouse monoclonal [5E6/2] to PCNA
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab70472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    modifications
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

References for Anti-PCNA antibody [5E6/2] (ab70472)

This product has been referenced in:
  • Urulangodi M  et al. Local regulation of the Srs2 helicase by the SUMO-like domain protein Esc2 promotes recombination at sites of stalled replication. Genes Dev 29:2067-80 (2015). Read more (PubMed: 26443850) »
  • Mayfield JA  et al. Surrogate genetics and metabolic profiling for characterization of human disease alleles. Genetics 190:1309-23 (2012). Read more (PubMed: 22267502) »

See all 3 Publications for this product

Product Wall

Gracias por tu respuesta.
No hay ningún problema en reemplazaros el anticuerpo por otro diferente, nuestra garantía Abpromise contempla esta posibilidad.
Acabo de realizar el pedido, completamente gratuito del anticuerpo ab104232 como solicit...

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Muchas gracias por mandarnos más detalles del protocolo.
Lamento mucho los inconvenientes causados, y tal y como especifica nuestra garantía Abpromise, os enviaré un reemplazo, reembolso, o vale sin caducidad en compensación.
He comprobado qu...

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Muchas gracias por reenviar el cuestionario.
Para tratar de obtener una señal más intensa os recomiendo llevar a cabo un lisado de la fracción nuclear en lugar de toda la célula. Para ello, podéis usar un buffer como RIPA, adecuado para este propó...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"