Overview

  • Product name
  • Description
    Rabbit polyclonal to PCNA
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).

  • Positive control
    • Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U87 MG, NIH 3T3, JC, BCL1, PC12, A549 and SiHa whole cell lysates; HeLa cells; Human KYSE170 xenograft and Human breast carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab152112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

  • Function
    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similarities
    Belongs to the PCNA family.
  • Post-translational
    modifications
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localization
    Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Images

  • PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 µg ab152112. Western blot was performed from the immunoprecipitate using ab152112.

    Lane 1: PCPB2 protein only. 

    Lane 2: IgG instead of ab152112 in HeLa whole cell extract. 

    Lane 3: ab152112 IP in HeLa whole cell extract. 

  • Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution.
  • Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.

    Counterstain is Hoechst 33342 (blue).

  • All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution

    Lane 1 : HCT116 whole cell lysate at 15 µg
    Lane 2 : HCT116 whole cell lysate at 10 µg
    Lane 3 : HCT116 whole cell lysate at 5 µg
    Lane 4 : HCT116 whole cell lysate at 1 µg


    Predicted band size : 29 kDa
    12% SDS PAGE
  • All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : 293T whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : HeLa whole cell lyate
    Lane 6 : HepG2 whole cell lysate
    Lane 7 : H1299 whole cell lysate
    Lane 8 : HCT116 whole cell lysate
    Lane 9 : MCF7 whole cell lysate
    Lane 10 : NT2D1 whole cell lysate
    Lane 11 : PC3 whole cell lysate
    Lane 12 : U87 MG whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    12% SDS PAGE
  • All lanes : Anti-PCNA antibody (ab152112) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    12% SDS PAGE
  • Anti-PCNA antibody (ab152112) at 1/1000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size : 29 kDa
    12% SDS PAGE
  • All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : NIH 3T3 whole cell lysate
    Lane 3 : PC12 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    12% SDS PAGE
  • Anti-PCNA antibody (ab152112) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 29 kDa
    12% SDS PAGE

References

ab152112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152112.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up