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Recombinant full length protein corresponding to PCNA.
Database link: P12004
Our Abpromise guarantee covers the use of ab15497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22238670
Fix with acetone.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
No special pretreatment required but maybe performed for optimum results in immunohistochemical staining of formalin/paraffin tissues.
ab15497/ab> staining PCNA in Human tonsil tissue (ab29614) by Immunohistochemisty (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab15497/ab> stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15497/ab>, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.